Fig. 5

Mitophagy regulates the intracellular survival of Mtb through BNIP3. A The percentage of apoptotic cells was determined in BNIP3-KO RAW 264.7 cells infected with Mtb (MOI = 1) for 24 and 48 h (n = 3 per group). B, C BNIP3-KO RAW 264.7 cells and siRNA (siControl or siBNIP3)-treated BMDMs were infected with Mtb (MOI = 1) for 24 and 48 h, and the intracellular survival was determined by counting the number of colony-forming units (CFU) (n = 9 per group). D, E WT or BNIP3-KO cells were treated with MitoTEMPO (100 μM) and then infected with Mtb (MOI = 1) for 24 and 48 h (n = 9 per group). Intracellular survival analysis was performed as described in B, C. The data are presented as means ± SD of least three independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001