Fig. 2
BNIP3 expression is induced via HIF1α activation in Mtb-infected macrophages. A BMDMs were infected with Mtb at an MOI of 1. The levels of HIF1α and BNIP3 were evaluated using western blotting. B BMDMs were pretreated with FM19G11 (20 μM) for 1 h and then infected with Mtb (MOI = 1) for 3 h. Cell lysates were collected 24 and 48 h post infection, western blotting was performed, and blots were probed using antibodies against HIF1α, BNIP3, and β-actin. C BMDMs were infected with Mtb (MOI = 1) for 48 h, and the levels of ROS were measured using a DHE stain (n = 5 per group). D BMDMs were pretreated with the ROS scavenger NAC (20 mM) for 1 h and then infected with Mtb at an MOI of 1 for 24 and 48 h. BMDMs treated with CoCl2 (200 μM) for 24 h were used as the positive control. Protein levels were analyzed by western blotting. β-actin was used as a loading control. Representative blots of three independent experiments are shown. The data are presented as means ± SD of least three independent experiments; **p < 0.01