Fig. 1
Mtb infection induces BNIP3 expression in macrophages. A, B Bone marrow-derived macrophages (BMDMs) were infected with Mtb at a multiplicity of infection (MOI) of 1 for 24 and 48 h. A Bnip3 mRNA was analyzed using quantitative real-time PCR. B Levels of BNIP3 at 24 and 48 h post infection were evaluated using western blotting. C BMDMs were infected with Mtb (MOI = 1 or 5) for 24 and 48 h. D BMDMs were infected with live or heat-killed (HK) Mtb (MOI = 1) for 24 and 48 h. E BMDMs were treated with ESAT-6 (10 μg/ml) or Mtb (MOI = 1) for 24 and 48 h, and expression of BNIP3 was evaluated using western blotting. BMDMs were treated with CoCl2 (200 μM) for 24 h and used as a positive control. β-actin was used as a loading control. Data are representatives from at least three independent experiments (mean ± SD of n = 4 in A); *p < 0.05