Fig. 9

Lipidomic analyses show reduced sphingomyelin species in Avil-ChR2 brain and plasma. A Volcano plot of the log2 difference (fold change) of lipids in the brain (quantified as AUC/IS) versus the negative logarithm of the t-test P-value. Prominent spots are labelled with the lipid name. Blue lipids were reduced in Avil-ChR2 mice, red lipids were increased. The data are of n = 15 ChR2-flfl and n = 16 Avil-ChR2 female mice. The inserts show scatter plots of regulated sphingomyelins (SM; reduced, blue) and lysophosphatidylcholine (LPC(24:0), LPC(24:1)); red, increased). For scatter plots, lipids were normalized to range 0.1. B In analogy to A the Volcano plots show lipids in plasma (quantified as AUC/IS). Blue labeled lipids were reduced in Avil-ChR2 mice. C, D Score plot and variable importance (VIP) plot of Partial Least Square (PLS-DA) analysis of brain lipids. The features’ importance agrees with the ranking according to t-test P values