Fig. 3

RNA sequencing of the brain cortex in Early-Life-Pain mice at P7. ChR2-flfl and Avil-ChR2 mice were exposed to blue light in a chamber on postnatal day P1-P5 together with the Cre-negative blue-insensitive mother. At P7, mice were euthanized and brain cortices subjected to transcriptome analysis via mRNA sequencing (n = 8 pups per genotype). The overview Volcano plot is shown in Additional file 1: Figure S1A. The abbreviations of the genes are the official gene symbols. Normalized counts of the presented genes along with gene description and GO terms are included as a Additional file 2: “Gene information” Excel file. RNAseq data have been deposited to the GEO database with the accession GSE200140. A Scatter plots of top candidate genes sorted according to gene abundance (normalized reads) and P-value. B: Scatter plots of genes with GO annotation “immune” or “inflammation”, P-value < 0.1 and Log2 difference < − 0.2 (down) or > 0.2 (upregulated). Low expression genes were filtered out. Each scatter is one mouse. Gene expression was compared per FDR adjusted t-test, and asterisks show the q-values. *q < 0.05, **q < 0.01, ***q < 0.001