Fig. 2
Cortical hyperexcitability in Early-Life-Pain mice at P7-8. Spontaneous cortical network activity of acute brain slices of ChR2-flfl and Avil-ChR2 mice at P8-9 after exposure to blue light on postnatal day P1-P5. A Photograph of an acute brain slice located on top and in touch with the 60-recording electrode MEA-chip setup. The gray square represents the recording area within the somatosensory cortex. B1 Representative voltage traces at one of the recording electrodes of the MEA in somatosensory cortex of ChR2-flfl (top trace) and Avil-ChR2 (lower trace). Note the higher number of spike events in the Avil-ChR2 group (lower voltage trace). B2 Inset showing one spike event at higher magnification (scale bar = 50 µV/1 ms). C1: Summary diagram showing the number of MEA electrodes for each experimental group, where multi-unit activity (MUA) could be detected in somatosensory cortex. C2: Summary diagram showing the frequency of the observed MUA in all active channels of ChR2-flfl and Avil-ChR2 slices (n = 10 mice per genotype). Each datapoint represents data from one brain slice. The data were statistically evaluated by an unpaired, 2-sided Student’s t-test. *P < 0.05. Transcriptomics reveal lower cortical expression of synaptic genes