Fig. 1

Schedule of Early-Life-Pain experiments and body weight time courses. A Schedule of blue light exposure of mice on postnatal day P1-P5, tissue and electrophysiology studies at P7-P8, behavior in adult mice (nociception, mazes, motor, IntelliCage) and DRG calcium imaging. B Time course of body weights. The scatters show body weight monitoring of n = 18 ChR2-flfl and n = 24 Avil-ChR2 female mice. The categorization into age classes considered behavioral experiments (e.g. before/after measurements) and data were analyzed via 2-way ANOVA for repeated measurements and subsequent posthoc analysis for each age class using an adjustment of alpha according to Šidák. The asterisks show adjusted P-values. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. C 24 h-feeding and drinking in Phenomaster cages of ChR2-flfl and Avil-ChR2 female mice at 50 weeks of age. The weights of feeding basket and drinking bottle were controlled with precision scales and were compared with unpaired, 2-sided Student’s t-test, n = 15 ChR2-flfl and n = 18 Avil-ChR2. The box is the interquartile range, the line the median and whiskers show minimum to maximum. No difference between genotypes