Fig. 7
Overexpression of SHP aggravated hepatic ischemia–reperfusion injury in mice with hepatocellular specific knockout of Sox9. Sox9 f/f and Alb-CreERT2/+; Sox9 f/f mice pre-treated with tamoxifen and indicated vectors were subjected to 1 h of hepatic ischemia. Plasma and liver samples were collected at 6 h post reperfusion. A Schematic diagram showing ischemia reperfusion model. B Sox9 and SHP mRNA expression were determined by qRT-PCR in indicated mice. C Plasma ALT level was measured in indicated group. TBA level was detected in livers and plasma in indicated group. TNFα and IL-6 mRNA expression were determined by qRT-PCR in indicated group. D Representative liver sections stained with H&E. Arrows, cell apoptosis; Dashed box, necrotic area. Scale bar, 200 µm. E Quantitative ROS detection in liver samples. F ROS production in frozen liver tissues from Sox9 f/f and H-Sox9 KO mice following IR. Scale bar, 100 μm. G Representative liver sections stained with TUNEL. Scale bar, 100 μm