Fig. 6

Overexpression of SOX9 reduces SHP nuclear localization and induces mitochondrial injury. Huh-7 cells were transfected with either SOX9 overexpression plasmid (SOX9 OE) or vehicle plasmid (Control). Then the cells were treated with 400 μM H₂O₂ for 6 h before harvest. A qRT-PCR was used to determine SOX9 and SHP mRNA expression in different cellular fractions in cell samples from indicated group. N = 3. B The subcellular localization of SHP in Huh-7 cells was observed under a fluorescence microscope. Scale bar, 50 µm. C Mito Tracker Red staining of Huh-7 cells treated with indicated concentration of H₂O₂. Scale bar, 50 µm. D Ca²⁺ indicator X-Rhod-1 in Huh-7 cells treated with vehicle or H₂O₂. Scale bar, 50 µm