Fig. 5
Loss of Sox9 in hepatocytes inhibits IR-induced cell death associated with SHP. Sox9 f/f and Alb-CreERT2/+; Sox9 f/f mice pre-treated with tamoxifen were subjected to 1 h of hepatic ischemia, liver samples were collected 6 h after reperfusion. A The representative images of TUNEL staining in liver tissue sections from Sox9 f/f and H-Sox9 KO mice with or without IR. Scale bar, 50 µm. B Western blot analysis on the expression levels of apoptosis related proteins Bax, Cyt C, Bid and Caspase-3 p17 (Caspase-3) in liver tissues. GAPDH was used as an internal control. C Quantification analysis of apoptosis related proteins Bax, Cyt C, Bid and Caspase-3. N = 3. D ROS production in frozen liver tissues from Sox9 f/f and H-Sox9 KO mice following hepatic IR. Scale bar, 100 µm. E Quantitative ROS detection in liver samples