Fig. 4
Hepatocyte-specific Sox9 deletion promotes SHP accumulation in nucleus. Sox9 f/f and Alb-CreERT2/+; Sox9 f/f mice pre-treated with tamoxifen were subjected to 1 h of hepatic ischemia. Liver samples were collected 6 h after reperfusion. A qRT-PCR was used to determine Sox9 and SHP mRNA in different cellular fractions in liver samples from Sox9 f/f and H-Sox9 KO mice. N = 3. B Western blot was used to determine SHP protein expression in different cellular fractions in liver samples. C Band intensities were measured by Image J. D Immunofluorescence co-staining for Sox9 and SHP. Scale bar, 50 µm. E The subcellular localization of SHP in hepatocytes was observed using confocal laser scanning microscopy. Scale bars, 50 µm. F Transmission electron microscopy analysis for hepatic mitochondrial morphology in Sox9 f/f and H-Sox9 KO mice induced by IR