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Fig. 7 | Cell & Bioscience

Fig. 7

From: Down-regulated FTO and ALKBH5 co-operatively activates FOXO signaling through m6A methylation modification in HK2 mRNA mediated by IGF2BP2 to enhance glycolysis in colorectal cancer

Fig. 7

FTO and ALKBH5 regulates HK2 dependent on IGF2BP2. A HK2 mRNA was detected by QPCR after IGF2BP1/2/3 knockdown. B RIP-QPCR was performed by IGF2BP2 protein and HK2 mRNA. C IGF2BP2, HK2 and FOXO1 protein levels were detected by western blot in IGF2BP2 knockdown cells. D–F CCK8 and colony formation assays were performed in IGF2BP2 down-regulation cells. G–I Cell migration and invasion was analysed in IGF2BP2 deficient HCT116 cells. J–L Both CCK-8 and colony formation assays indicated the ability of proliferation in FTO/ALKBH5 silence HCT116 cells was restored by IGF2BP2 knockdown. M–O Analysis of cell migration and invasion while transfected with shIGF2BP2 in FTO/ALKBH5 deficient HCT116 cells (magnification times, 100 ×). **p < 0.01; ***p < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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