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Fig. 5 | Cell & Bioscience

Fig. 5

From: Down-regulated FTO and ALKBH5 co-operatively activates FOXO signaling through m6A methylation modification in HK2 mRNA mediated by IGF2BP2 to enhance glycolysis in colorectal cancer

Fig. 5

Down-regulation of FTO and ALKBH5 facilitates CRC progression via activating FOXO1 mediated by HK2 A, B The mRNA and m6A levels of FTO knockdown and over-expression in HCT116 cells. C, D The mRNA and m6A levels of ALKBH5 knockdown and over-expression in HCT116 cells. E, F The HK2 expression was measured by RT-PCR in FTO knockdown/over-expression, and ALKBH5 knockdown/over-expression in HCT116 cells was verified by qPCR. G Western blot showing HK2 and FOXO1 expression in CRC cells treated with FTO knockdown/over-expression, ALKBH5 knockdown/over-expression in HCT116. H–K CCK-8, colony formation, and EdU assays all indicated the ability of proliferation in FTO/ALKBH5 deficient CRC cells was rescued by HK2 knockdown. L–N Analysis of CRC cell migration and invasion while transfected with shHK2 in FTO/ALKBH5 deficient CRC cells.*p < 0.05; **p < 0.01; ***p < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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