Fig. 7

Fibroblast lactate controls cardiomyocyte hypertrophy. A, Echocardiographic analysis of WT and MCT1 CKO mice 4 weeks after saline or Ang II infusion. B Heart weight/body weight (mg/g) ratio of four groups of mice n ≥ 4/group. C through F Quantification of echocardiography parameters: LVPWD (C), IVSs (D) and IVSd (E), LVEF (F). G Representative images of haematoxylin and eosin (H&E) staining, Masson’s staining, immunohistological images of Col I-positive cells in hearts and images after WGA staining. H Quantification of collagen volume fraction (n = 5/group). I Quantification of the relative cross-sectional area (n = 4/group). J, K, Representative immunoblot images showing a-SMA and vimentin protein expression in hearts (J). The quantification of these proteins (K) (n ≥ 3/group). L, M, Representative immunoblot images showing ANP and MYH7 protein expression in hearts (L). The quantification of these proteins (M) (n ≥ 3/group). The data are shown as the mean ± SEM. P values were calculated by one-way ANOVA. ^*p < 0.05, ^**p < 0.01