Fig. 5

Increased lactate flux capacity during CFs differentiation. A and B, Images of CFs stained with immunofluorescent pH (potential of hydrogen) probe (red) and Hoechst (blue). Scale bar = 100 μm (A). The relative immunofluorescence intensity (B) (n = 10/group). C Relative quantification of the lactic acid concentration in the cell medium (n ≥ 6/group). D and E CFs were treated with or without Ang II after transfection with an empty vector or sh-GCN5L1. Representative immunoblot images showing LDHA and MCT4 protein expression (D). The quantification of these proteins (E) (n ≥ 3/group). F and G CFs were treated with Ang II after transfection with an empty vector or LV-GCN5L1. Representative immunoblot images showing LDHA and MCT4 protein expression (F) (n ≥ 3/group). The quantification of these proteins (G). H and I Representative images of CFs treated with or without Ang II after transfection with an empty vector or sh-MPC2 stained with an immunofluorescent pH probe (red) and Hoechst (blue). Scale bar = 100 μm (H). Quantification of the relative fluorescence intensity (I) (n ≥ 9/group). J and K Representative immunoblot images showing LDHA and MCT4 protein expression in CFs treated with or without Ang II after transfection with an empty vector or sh-MPC2 (J). The quantification of these proteins (K) (n ≥ 3/group). L and M Representative images of CFs treated with or without Ang II after transfection with an empty vector or sh-LDHA stained with immunofluorescent a pH probe (red) and Hoechst (blue). Scale bar = 100 μm (L). Quantification of the relative fluorescence intensity (M) (n = 9/group). The data are shown as the mean ± SEM. P values were calculated by one-way ANOVA. *p < 0.05, **p < 0.01