Fig. 4

GCN5L1 interacts with and acetylates MPC2. A Immunofluorescence assay showing colocalization of GCN5L1 and MPC2 in cellular mitochondria. Scale bar = 50 μm. B and C Representative western blot images of 293T cell extracts immunoprecipitated with GCN5L1 (B) or MPC2 (C) and subsequently immunoblotted with anti-MPC2 and anti-GCN5L1 antibodies. D and E 293T cells transfected with Flag-GCN5L1 (D) or Flag-MPC2 (E), which was pulled down with anti–Flag beads and subsequently immunoblotted with an anti-GCN5L1 or anti-MPC2 antibody. F and G immunoblot images showing the Ac-MPC2^K19 protein expression in CFs treated with Ang II after infection with an empty vector or sh-GCN5L1 (F). The quantification of this protein (G). n = 3/group. H and I immunoblot images showing the Ac-MPC2^K19 protein expression in CFs treated with or without Ang II after infection with an empty vector or LV-GCN5L1 (H). The quantification of this protein (I). n = 3/group. J and K Representative immunoblot images showing Col I, a-SMA and vimentin protein expression in CFs transfected with Flag-MPC2^WT, Flag-MPC2^K19Q or Flag-MPC2^K19R (J) and the quantification of these proteins (K) (n = 4/group). L and M immunoblot images showing MPC2, a-SMA and vimentin protein expression in CFs treated with or without Ang II after transfection with an empty vector or sh-MPC2 (L). The quantification of these proteins (M) (n ≥ 3/group). The data are shown as the mean ± SEM. P values were calculated by one-way ANOVA. ^*p < 0.05, ^**p < 0.01