Fig. 2

GCN5L1 is critical for the fibroblast-to-myofibroblast transition. A and B Representative images showing GCN5L1, a-SMA, Postn, and vimentin protein levels in CFs with or without Ang II treatment after transfection with an empty vector or GCN5L1-interfering lentiviruses (sh-GCN5L1) (A). The quantification of these proteins (B) (n = 4/group). C and D Representative immunoblot images showing GCN5L1, a-SMA, Postn and vimentin protein levels in CFs with or without Ang II after transfection with an empty vector or overexpressing lentiviruses (LV-GCN5L1) (C). The quantification of these proteins (D) (n = 4/group). E and F Representative images of a-SMA (red) and DAPI (4’,6-diamidino-2-phenylindole; blue) immunofluorescence. Scale bar = 100 μm (E). Quantification of the relative fluorescence intensity (F). G and H Representative images showing the cell wound healing assay (scale bar = 200 μm) (G) and quantification of the percentage of the wound closed (H) (n ≥ 8/group). I and J Representative images showing transwell migration and invasion experiments (scale bar = 200 μm) (I) and quantification of the number of migrated cells (J) (n = 8/group). The data are shown as the mean ± SEM. P values were calculated by one-way ANOVA. ^*p < 0.05, ^**p < 0.01