Fig. 1

Loss of GCN5L1 attenuates cardiac fibrosis and hypertrophy. A, Echocardiographic analysis of WT and GCN5L1 CKO mice 4 weeks after saline or Ang II infusion. B, Heart weight/body weight (mg/g) ratio of four groups of mice(n ≥ 8/group). C, D and E, Quantification of echocardiography parameters LVAWD, LVPWD and LVEF(n ≥ 8/group). F, Representative images showing haematoxylin and eosin (H&E) staining (scale bar = 2 mm), Masson’s staining, immunohistology staining of Col I-positive cells in hearts and images showing WGA staining (scale bar = 100 μm). G, Quantification of collagen volume fraction(n = 11/group). H, Quantification of the relative cross-sectional area (n = 10/group). I and J, Representative immunoblot images showing ANP, BNP and MYH7 protein levels in hearts (I). The quantification of these proteins (J) (n = 3/group). K and L, Representative immunoblot images showing Col I, a-SMA and vimentin protein levels in hearts (K). The quantification of these proteins (L) (n = 3/group). The data are shown as the mean ± SEM. P values were calculated by one-way ANOVA. ^*p < 0.05, ^**p < 0.01