Fig. 6

PGAM5 silencing suppressed antioxidative genes in HepG2 cells. HepG2 cells were stimulated with oleic acid (OA) and palmitic acid (PA) and treated with NC or PGAM5-siRNA for 24–48 h. a TG levels measured in HepG2 cells. b, c Relative protein levels of p-mTOR/t-mTOR, p-InsR/t-InsR and p-AKT/t-AKT measured by Western blot. d, e Relative protein levels of antioxidative proteins including NRF2, CAT, HO-1, GPX6 and SOD1 measured by Western blot. *p < 0.05, statistical differences between NC and siPGAM5 group. ns, no significance; mTOR, mammalian target of rapamycin; InsR, insulin receptor; AKT, protein kinase B; NRF2, nuclear factor erythroid 2- related factors; CAT, catalase; HO-1, heme oxygenase-1; GPX6, glutathione peroxidase 6; SOD1, superoxide dismutase 1; NC, negative control; siPGAM5, PGAM5-knockdown