Fig. 4

Knockout of PGAM5 shows no protective effect in mice fed with MCS or MCD diet. a–f Body weight (a), liver TG (b), serum AST (c) and ALT (d), serum levels of TNF-α (e) and IL-6 (f) were examined in mice fed with MCS or MCD diet for six weeks. g–k Representative H&E staining of liver sections and evaluation of NAS (including scores of steatosis, inflammation and ballooning injury). Scale bars, 100 μm. l, m Representative PSR staining of fibrosis in liver sections and its statistics of positive area (%). Scale bars, 50 μm n, t Relative hepatic protein levels of antioxidative genes including GPX6 (o), HO-1 (p), CAT (q), NRF2 (r), SOD1 (s) and NRF1 (t) measured by Western blot. N = 9–11; *p < 0.05, **p < 0.01, statistical differences between WT and GKO group. ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001, statistical differences between diets, genotypes or their interactions. TG, triglyceride; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GPX6, glutathione peroxidase 6; HO-1, heme oxygenase-1; CAT, catalase; SOD1, superoxide dismutase 1; NRF2, nuclear factor erythroid 2- related factors; NRF1, nuclear respiratory factor 1; MCS, methionine choline supplemented; MCD, methionine choline deficient; WT, wild type; GKO, global knockout