Fig. 3

PUS10 inhibits the migration of RCC in a catalytic-independent manner. A Overexpression of PUS10 was verified by qRT‒PCR using GAPDH as a reference. B Western blot and dot blot assay were performed to verify the construction of wildtype and mutant PUS10 overexpressing models in vitro. C Transwell assays were conducted to evaluate the migration inhibition effect of wild-type and catalytically incompetent PUS10. Migrating cells in three replicate experiments were counted and are presented as the mean ± SD in the histogram. D Wound healing assay validates the similar impact of wild-type and mutant PUS10 ectopic expression on the migratory ability of RCC cells. E Transwell assays showed that supplementation with either wild-type or mutant PUS10 could reverse the promotion of RCC migration induced by PUS10 silencing with siRNA. Migrating cells in three replicate experiments were counted and are presented as the mean ± SD in the histogram. *P < 0.05, **P < 0.01, ***P < 0.001; ns not significant