Fig. 2
Silencing of PUS10 promotes RCC migration in vitro and in vivo. A qRT‒PCR assay revealed that PUS10 was knocked down in 786-O and Caki-1 cells after the transfection of siRNAs. Three independent experiments are shown as the mean ± SD, and GAPDH was used as a reference. B Representative western blot images showing the silencing efficiency of PUS10 in RCC cell lines. C Transwell assays and wound healing assays were performed to measure the migration ability in vitro after PUS10 depletion. Migrating cells in three replicate experiments were counted and are presented as the mean ± SD in the histogram. D Wound healing assay validated the promoted migratory ability of 786-O and Caki-1 cells after PUS10 knockdown. A representative of three replicated experiments is shown. The wound healing rate was calculated and presented as the mean ± S.D. E Luciferase-tagged ACHN cells with or without PUS10 knockdown were constructed and injected into the tail vein of nude mice. Bioluminescent images were taken after 8 weeks with signal intensities (photons/s/cm2/sr) qualified using the IVIS system. *P < 0.05, **P < 0.01, ***P < 0.001; ns not significant