Fig. 9
From: APP family member dimeric complexes are formed predominantly in synaptic compartments
Expression analysis of APP, APLP1, and APLP2 dimers in mouse cortices in synaptosomal preparations. A, C, E WT mouse (C57BL/6) brain homogenates were sub fractionated by differential centrifugation steps and analyzed by Western blot with the primary α-APP C-terminal antibody Y188, α-APLP1 antibody 57, and α-APLP2 D2-II antibody. α-Synaptophysin and α-PSD-95 antibodies, respectively, were used as presynaptic and postsynaptic markers. To confirm specificity of the signals, synaptosome preparations were also performed with APP, APLP1, and APLP2 KO mouse brains. B, D, F Preparation of the post nuclear fraction (PNF) and the concentrated synaptosomes (Syn H-co) under semi-denaturing conditions for analysis of dimers on Blue Native gels. Western blot detection of APP followed with the primary α-APP C-terminal antibody Y188 (B), of APLP1 with the primary α-APLP1 antibody 57 (D) and APLP2 with the primary α-APLP2 antibody D2-II (F)