Fig. 11

Alzheimer’s disease patients show decreased amounts of APP dimers in the brain. A Samples of the middle frontal gyrus of AD patients and human controls, age matched, and area matched, were lysed under semi-denaturing conditions for analysis on Blue Native gels. Western blot detection of APP followed with the primary α-APP C-terminal antibody Y188. B Quantification of data shown in A. Bars represent mean values ± SEM of monomer/dimer ratio to the total amount of the signal in %. n = 5 (human controls), n = 6 (AD brains), unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001. C Samples of the middle frontal gyrus of AD patients and human controls, age matched, and area matched, were lysed in buffer containing 1% NP40 for analysis on SDS gels. Western blot detection of APP followed with the primary α-APP C-terminal antibody Y188 and an α-β-actin antibody was used to detect β-actin protein levels as a loading control