Fig. 1

Comparative analysis of FKBP induced dimerization of the APP gene family members. A Schematic illustration of the used constructs APP F1, APLP1 F1 or APLP2 F1. By addition of the rapamycin analogue AP20187 the FKBP tags can be dimerized. B The FKBP tagged APP gene family members were heterologously expressed in N2a cells. Non-transfected N2a cells served as a negative control. Equal amounts of cell lysates were separated on denaturating SDS-Gels and Western blot detection followed with an α-HA antibody. Detection with primary antibody β-actin was performed as a loading control. C APP F1, APLP1 F1 or APLP2 F1 were heterologously expressed in N2a cells and treated with 100 nM of the rapamycin analogue AP20187 overnight to induce dimerization of APP/APLPs. Incubation with the solvent of AP20187, ethanol, served as a negative control. The samples were analyzed via semi-denaturing Blue Native PAGE and Western blot analysis followed with an α-HA antibody