Fig. 3
Enhanced anti-tumor activity by the functional immune system on Il1rn knockout (KO)-derived tumors. (A) Images of tumors collected from the pancreas of immune-competent C57BL/6JNarl (B6) mice after orthotopic injection with 6606PDA-derived cell lines. No tumors were detected in mice injected with #E clone. (Ctrl: n = 5; KO clones: n = 3) (B) Statistical analysis of tumor weights from panel A. (C) Comparison of body weights between Ctrl and #A clone in another set of orthotopic injected C57BL/6NCrlBltw (B6’) mice (Ctrl: n = 5; #A: n = 10). Two mice injected with Ctrl cells died during the experiment. (D) Statistical analysis of tumor weights from panel C. (E) Analysis of tumor growth in advanced severe immune deficient (ASID) mice. Images of tumors collected from the ASID mice orthotopically injected with tumor cells (upper panel). Tumor weights were measured and compared (lower panel). (F) Comparison of IL1RN KO-dependent inhibitory effects between ASID and B6 mice. Data in panel B and panel E were analyzed following the formula: 1-weightKO/weightCtrl. Ctrl: parental 6606PDA cells. #A~#E: IL1RN-KO 6606PDA cells. Student’s t-test: * p < 0.05. ** p < 0.01. *** p < 0.001