Fig. 4

c-Myc promotes PKM2 expression via hnRNPs isoforms. A Schematic illustration of how c-Myc increases PKM2 expression via hnRNPs isoforms. B–C Western blot analysis of the cytoplasmic and nuclear expression of c-Myc in CaCo2/EV and CaCo2/KITENIN cells. D hnRNPI (PTBP1), hnRNPA1, and hnRNPA2 mRNA expression in CaCo2/EV and CaCo2/KITENIN cells. E–F hnRNPA1 and hnRNPA2 protein level in CaCo2/EV and CaCo2/KITENIN cells. G hnRNPI (PTBP1), hnRNPA1, and hnRNPA2 mRNA expression in KITENIN-silenced cells. H–I KITENIN knockdown reduces the protein level of hnRNPA1 and hnRNPA2 in CaCo2/EV cells. J Knockdown of KITENIN and MYO1D inhibit c-Myc and hnRNPI, hnRNPA1, hnRNPA2 mRNA levels on PKM2-myc transfected cells. CaCo2 cells were transfected with si-KITENIN and si-MYO1D for 24 h followed by transfection of the plasmid, a construct expressing myc-tagged PKM2 (PKM2-myc) for 24 h and subjected to the qRT-PCR. Data are mean ± standard deviation, n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001, NS, no significant difference between groups. (K–M) The GEPIA web tool was used to analyze the relationships of the mRNA expression of KITENIN with that of hnRNPI (PTBP1), hnRNPA1, and hnRNPA2 in COAD samples