Fig. 2

KITENIN knockdown reduces the expression of molecules responsible for the Warburg effect. A–B CaCo2/EV and CaCo2/KITENIN cells were transfected with si-KITENIN and then their metabolism was assessed. The extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured using a Glycolytic Rate Assay Kit on a Seahorse XF96 extracellular flux analyzer. To measure glycolytic rate, the assay utilizes both ECAR and OCR measurements to determine the glycolytic proton efflux rate (glycoPER). GlycoPER was measured at two time points, and then following the sequential injection of rotenone (1 μM)/antimycin A (1 μM) and 2-DG (50 mM). C Effect of si-KITENIN on the mRNA expression of KITENIN and ErbB4. D–F Relative mRNA expression and protein level of molecules mediating the Warburg effect (GLUT1, HK2, PKM1, PKM2, and LDHA) in CaCo2/EV cells transfected si-negative control or si-KITENIN for 48 h. G–H CaCo2/EV and CaCo2/KITENIN were transfected with si-MYO1D and then their metabolism was assessed. The extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured using a Glycolytic Rate Assay Kit on a Seahorse XF96 extracellular flux analyzer. To assess the glycolytic rate, the assay utilizes both ECAR and OCR measurements to determine the glycolytic proton efflux rate (glycoPER). GlycoPER was measured at two time points, and then following the sequential injection of rotenone (1 μM)/antimycin A (1 μM) and 2-DG (50 mM). I–J Relative mRNA expression of KITENIN, ErbB4, GLUT1, HK2, PKM1, PKM2, and LDHA in CaCo2/EV cells for incubated 48 h after transfection with si-MYO1D. Data are mean ± standard deviation. * p < 0.05; ** p < 0.01; *** p < 0.001, NS, no significant difference between groups