Fig. 2
From: SOX9 is a key component of RUNX2-regulated transcriptional circuitry in osteosarcoma
SOX9 is a survival factor of OS cells in vitro. A, Immunoblotting showing the protein levels of SOX9 and RUNX2 across different OS cell lines and mesenchymal stem/stromal cells (MSCs). B and D, Immunoblotting showing SOX9, cleaved caspase 3, and β-actin levels in SAOS2 (B) and HOS-MNNG (D) cells 6 days after SOX9 knocdown. C and E, Propidium iodide staining showing the effect of SOX9 knockdown on cell cycle and apoptosis (sub-G1) in SAOS2 (C) and HOS-MNNG (E) cells 6 days after the knockdown