Fig. 4

Inhibition of glutathione metabolism or ferroptosis reverses cisplatin-induced death of OCCC cells, as determined in vitro. (A) TOV21G or OVCAR3 cells were treated with the indicated concentrations of CB-839 with cisplatin. Cell viability was measured by CCK8 assay 72 h after treatment. (B) TOV21G or OVCAR3 cells were treated with the indicated concentrations of liproxstatin-1 with cisplatin. Cell viability was measured by CCK8 assay 72 h after treatment. (C-D) TOV21G/OVCAR3 cells were treated with CB-839 (10 𝛍M) and liproxstatin-1 (10 𝛍M) with cisplatin for 72 h. Representative images and summary of the percentage of Annexin V-positive cells as determined by flow cytometry

NT: no treatment. All assays were carried out in triplicate, and the data are presented as the mean ± S.D. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001