Fig. 2
CLSM images of Aβ amyloidogenic aggregates in a brain tissue section from a 17 months old 3×TgAD mouse. A Large-capture tile & stich CLSM image of a sagittal mouse brain section showing Aβ amyloid aggregates in the hippocampus (left) and the cerebral cortex (right), visualized using 1C3-DyLight633. Scale bar 2 mm. B Magnified CLSM image of the rectangular area highlighted in A. Different regions selected for super-resolution STED microscopy imaging (shown in Fig. 3) indicate: a small plaque (1), plaque outskirt (2) and a detail of a large plaque (3). Scale bar 10 μm. C Immunohistochemical control obtained by displacing 1C3-DyLight633 by simultaneous incubation with unlabelled Aβ40 in large excess, (roughly 45 or 50 × higher). The average photon count in the background shown in C, PCbg ≈ 7 photons, is the same as the background in B, indicating that: (1) nonspecific binding of 1C3-DyLight633 is low and (2) regions characterized by fluorescence > PCbg indicate the presence of Aβ monomers/small-sized oligomers that could not be resolved by STED imaging. Scale bar 10 μm