Table 1 List of genes and proteins’ phosphorylation affected by TEPA in RNA-Seq, Real-Time PCR, and western blot analysis of MDA-MB-231, SH-SY5Y, DIPG007, and DIPG010 cell lines. These genes and proteins are important in the migration, and invasion of cancer cells via dysregulation of TGF-β and EMT pathways
Cell lines | GenesIprotein symbol | |
|---|---|---|
Downregulated | Upregulated | |
MDA-MB-231 | TGF-β1, TGFBR2, SPARC, SNAI2, SMAD2, p-SMAD2, MMP-2, MMP-9, MMP-14, MMP- 11, MMP-15, Vimentin, RAC2, BCL2, PLOD2, PLAT, MARCKSLl, CCR7, PAK l, ZYX, LAMA4, p-AKT, p-ERK l/2 | CDKN1A, TIMP3, TIMP4, E-cadherin, (CDH l) |
SH-SY5Y | TGF-β1, p-SMAD2, SMAD2, MMP-9, MMP-14, Vimentin, ZEBl, ZYX, RACl, PLAT, p-AKT, p-ERK l/2 | TGIFl, CDKN1A |
DIPG007 | SMAD2, p-SMAD2, MMP-2, MMP-9, MMP-14, Vimentin, ZYX, VCAN, PXN, AKT, p-AKT, ERK l/2, p-ERK l/2 | CDKN1C |
DIPG010 | TGF-β1, SPARC, N-cadherin (CDH2), Vimentin, MMP-2, ZYX, LAMA4, PLAT, VCAN |