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Fig. 3 | Cell & Bioscience

Fig. 3

From: Copper chelation suppresses epithelial-mesenchymal transition by inhibition of canonical and non-canonical TGF-β signaling pathways in cancer

Fig. 3

a Confocal images (inverted for signal visibility) showing epithelial and mesenchymal cell state markers in MDA-MB-231 TNBC cells across control or treated conditions (TGF- β, CuCl2, TEPA or CuCl2 + TEPA). Segmented cell nuclei and cytoplasm outlines shown (red). All scale bars 20 μm. b Violin plots showing quantification of single cell mean fluorescence intensities. c Violin plots showing quantification of single cell nuclear-to-cytoplasmic (N:C) mean intensity ratio. Total cells per treatment condition: control = 21848; TGF- β = 17743; CuCl2 = 19153; TEPA = 25200; and CuCl2 + TEPA = 18705. 14000–23000 cells per marker, 2000–6000 cells per marker per treatment group. Results of two-sided Mann–Whitney-Wilcoxon testing with Bonferroni correction: ns = (5.00e-02 < p <  = 1.00e + 00), *: (1.00e-02 < p <  = 5.00e-02), **: (1.00e-03 < p <  = 1.00e-02), ***: (1.00e-04 < p <  = 1.00e-03), ****: (p <  = 1.00e-04)

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Cell & Bioscience

ISSN: 2045-3701

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