Fig. 8

A schematic summary of the present study. The schematic overview shows the mechanism of EDA-induced EDAR plasma membrane trafficking in both cell cultures and skin appendage formation. Our findings indicate that only a small portion of EDAR is located on cell membrane when no EDA appears and EDA signaling is blocked. Here, we find that EDA binding to plasma membrane EDAR initiates a pathway to exacerbate further plasma membrane trafficking of cytosolic EDAR. We also identify a HED-linked EDAR mutation T346M, which leads to the failure of EDAR translocation to plasma membrane. Our study thus provides a model of ligand-triggered plasma membrane trafficking of its own receptor for positive feedback regulation