Fig. 7

Either PKA activation or ectopic expression of SNAP23 augments EDA-dependent MG growth. A, C IHC staining of LEF1 (red) and K14 (green) in cultured EDA^−/− eyelids with indicated treatment. B, D Average length of MG germs in A and C. Error bars indicate mean ± SD from at least 20 MGs of total 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test. Scale bar, 20 μm