Fig. 6

Abnormal behaviors in Dgcr2 mt mice. A-D Open field test (OFT). Representative traces of mice in the OFT (A). Mice were placed in the chambers and movement was monitored for 10 min. The square with dotted line indicates the center area. Distances traveled in total (B) or center area (C) during 10 min was similar between wt and mt mice. Decreased movement time in center area in mt mice (D). n = 21 wt mice and 14 mt mice. E-G Elevated plus maze test (EPM). Diagram of EPM (E). Mice were put in the center of EPM and they can freely explore for 10 min. The time stayed in the open arms (F) and entries into the open arms (G) were recorded. n = 21 wt mice and 14 mt mice. H-K Contextual fear conditioning test (contextual FC). Diagram of contextual FC (H). Day 1, six foot-shocks (FS) were delivered (0.5mA, 2s) during training. Day 2 and day 3, mice were placed into the same chambers for 30 min and 5 min, respectively, and the freezing time was recorded. BS, baseline. Impaired fear acquisition in Dgcr2 mt mice after five FS (I). Similar fear extinction between mt and wt (J-K). n = 13 wt mice and 12 mt mice. L-N Prepulse inhibition test (PPI). Diagram of PPI (L). Response to auditory-evoked startle stimulus (120 dB) was measured (M). The mt and wt exhibited similar baseline of startle responses. Increased PPI in mt mice (N). n = 12 wt mice and 11 mt mice. * p < 0.05, Student’s t test