Fig. 3

Impaired glutamatergic transmission and synaptic plasticity in Dgcr2 mt mice. A Representative traces of mEPSCs in CA1 pyramidal neurons from wt and mt mice. Scale bars represent 4 s, 10 pA. B-E Cumulative probability plots of mEPSC inter-event intervals and histograms of mEPSC frequency (B and C) and amplitude (D and E). n = 18 neurons from 4 wt mice, n = 13 neurons from 4 mt mice. F Representative traces of mIPSCs in CA1 pyramidal neurons from wt and mt mice. Scale bars represent 4 s, 20 pA. G-J Cumulative probability plots of mIPSC inter-event intervals and histograms of mIPSC frequency (G and H) and amplitude (I and J). n = 15 neurons from 4 wt mice, n = 12 neurons from 4 mt mice. For C, E, H and J, student’s t test, * p < 0.05. K Representative sweeps with inter-stimulus interval of pair-pulse stimulations at 100 ms. Scale bars: 50 ms and 100 pA. L Paired-pulse ratios plotted against inter-stimulus intervals. n = 16 neurons from 4 mice for both genotypes. Two-way ANOVA, p = 0.074. M Depressed I/O curves in the hippocampus of mt mice. fEPSPs were recorded by stimulating the SC-CA1 pathway with gradual increasing intensities. n = 9 neurons from 4 wt mice, n = 10 neurons from 3 mt mice. Two-way ANOVA, p < 0.0001. N Impaired LTP at SC-CA1 pathway in the mt mice. Representative traces shown fEPSP indicated with 1 and 2. Scale bars represent 2 ms, 0.5 mV. O The maximum evoked fEPSP slope is measured during the first 10 min after induction. P The enhanced fEPSP slope is measured during the last 20 min of recording. For O and P, Student’s t test, * p < 0.05