Fig. 1

Localization of DGCR2 to the PSDs. A Colocalization of DGCR2 with MAP2. DIV19-20 hippocampal neurons were co-stained with anti-DGCR2 and anti-MAP2 (a dendrite marker) or anti-Tau-1 (an axon marker) antibody. Scale bar as indicated. B Schematic illustration of DGCR2 domain structure. SP, signal peptide; LDL, Low-Density Lipoprotein (LDL) receptor binding domain similarity; lectin, C-type lectin similarity; VWFC, von Willebrand factor (VWF) type C repeat; TM, transmembrane domain; ICD, intracellular domain; TVV, amino acids for threonine, valine, valine. C Binding of DGCR2 with PSD-95 in HEK 293T cells. Cells were co-transfected with PSD-95-EGFP or EGFP alone, and FLAG-hDGCR2 or FLAG-hDGCR2 ∆TVV. Lysates were immunoprecipitated with anti-GFP antibody and Protein A/G agarose, and resulting complexes were blotted with anti-GFP and anti-FLAG antibodies. Inputs were blotted for perspective proteins as controls. D Colocalization of DGCR2 with PSD-95 puncta. DIV19-20 hippocampal neurons were co-stained with anti-DGCR2 and anti-PSD-95 (a PSDs marker) antibodies. Scale bar as indicated. E Enriched expression of DGCR2 in the PSDs. Subcellular fractions of adult wt mouse brains were blotted with anti-DGCR2, anti-PSD-95 and anti-synaptophysin (a presynaptic marker). S1, supernatant 1; P1, pelleted nuclear fraction; P2’, washed crude synaptosomal fraction; S3, crude synaptic vesicle fraction; P3, lysed synaptosomal membrane fraction; SPM, synaptosomal membrane fraction; Pre, presynaptic fraction; PSD, postsynaptic density fraction