Fig. 8

Activation of AhR/Nrf2 signaling improves the LSECs damage induced by HSOS-derived fecal supernatant in vitro. A Cell viability measured using Cell Counting Kit-8 assay. B The protein expression of MMP-9 in LSECs by Western Blot analysis. C, D The nuclear AhR and CYP1A1 expression in LSECs. E The nuclear Nrf2 expression in LSECs. F The mRNA expression of GCLC, GCLM and NQO1 in LSECs. Data were shown as mean ± SEM. N = 4. *P < 0.05, **P < 0.01, vs. Norm-FSN group; #P < 0.05, vs. HSOS-FSN group. HSOS, hepatic sinus obstruction syndrome; FSN, fecal supernatant; Ficz, 6-Formylindolo[3,2-b]carbazole; MMP-9, matrix metalloproteinase-9; AhR, aromatic hydrocarbon receptor; Nrf2, nuclear factor E2-related factor 2; CYP1A1, cytochrome P450 1A1; GCLC, glutamate-cysteine ligase catalytic subunit; GCLM, glutamate-cysteine ligase modifier subunit; NQO-1, NAD(P)H dehydrogenase quinone 1; GPADH, glyceraldehyde-3phosphate dehydrogenase