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Fig. 2 | Cell & Bioscience

Fig. 2

From: Multiple Fra-1-bound enhancers showing different molecular and functional features can cooperate to repress gene transcription

Fig. 2

Repression of Pol II activity by Fra-1 at the TGFB2 locus. A TGFB2 gene and ChIP-qPCR amplicons. The positions of the amplicons used in ChIP-qPCR experiments are indicated in kb from the TSS. All experiments were carried out using MDA-MB-231 cells transfected with either siFra-1 (green boxes) or siCTL (violet boxes) for 72 h. All values were normalized to that of amplicon + 2.9 kb under control condition arbitrarily set to 1. The arrows indicate the TGFB2 TSS. B ChIP-qPCR analysis of total Pol II on the TGFB2 gene. Four independent experiments were conducted. C ChIP-qPCR analysis of Pol II-PSer5 on the TGFB2 gene. Four independent experiments were conducted. D ChIP-qPCR analysis of CDK7 on the TGFB2 gene. Five independent experiments were conducted

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Cell & Bioscience

ISSN: 2045-3701

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