Fig. 6

Umbelliferone represses the Omicron BA.2-associated pulmonary inflammation in vivo. A Schematics of the administration process of the mouse model. The mouse model was created by bioRENDER. The mice infected with VSVG or Omicron BA.2 pseudoviruses were sacrificed after oral administration with sterilized water (Ctrl), 70 mg/kg umbelliferone (UMB), and 10 mg/kg cepharanthine (CEP) for 9 days, and lungs were subsequently harvested for the examinations of histopathological changes in H&E staining B, lymphocyte infiltration and TNF-α protein expression in IHC staining B, TMPRSS2 protein in Western blot analysis C, and the mRNA levels of indicated genes in RT-qPCR D-K, respectively. * p < 0.05, ** p < 0.01, and *** p < 0.001