Fig. 4

Calcineurin inhibition protects against MPTP induced behavioral deficiency in presence/absence of L-DOPA while VIVIT peptide fails to offer any. A Treatment paradigm for MPTP induced Parkinson’s disease model generation. MPTP (30 mg/kg) is treated on day 1 and 2 (16 h apart). From 3rd day the mentioned treatments are initiated, alone or in combination. Behavior is evaluated on 7th, 8th, 14th and 15th day. On 8th and 15th day, behavioral assays are done after 16 h of the last treatment. Neurotransmitter level analysis and histochemistry are done on either 8th or 15th day. [*] in red denotes the day in which animals are sacrificed. B Akinesia and catalepsy on the mentioned days for the treatment groups are evaluated. At least 4 animals are analyzed for each experiment. *P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001 when compared to control group; ^#P ≤ 0.05, ^##P ≤ 0.01,^###P ≤ 0.001,^####P ≤ 0.0001 when compared to MPTP treated group. P values are calculated by two way ANOVA followed by Tukey’s multiple comparison test. C Trajectories from the mean swim score (± SEM) for the mentioned time is plotted and the cumulative score for 10 min, for each group on 8th and 15th day is represented. At least 4 animals are taken for the analysis. *P ≤ 0.05, **P ≤ 0.01,***P ≤ 0.001 when compared to the control group. P values are calculated by one way ANOVA followed by Tukey’s multiple comparison test. D Akinesia and catalepsy are evaluated on the mentioned days for the treatment groups. At least 4 animals are taken for the evaluation. *P ≤ 0.05, **P ≤ 0.01,***P ≤ 0.001, ****P ≤ 0.0001 when compared to control group; ^##P ≤ 0.01 when compared to MPTP treated group. P values are calculated by two way ANOVA followed by Dunnetts multiple comparison test. E Trajectories from the average swim score (± SEM) for the mentioned time is plotted and the cumulative score for 10 min, for each group on 15th day is represented. At least 4 animals are taken for the analysis. **P ≤ 0.01,***P ≤ 0.001 and **** P ≤ 0.0001 when compared to the control group. P values are calculated by one way ANOVA followed by Dunnett’s multiple comparison test. Bar graphs represent mean ± SEM