Fig. 2

Dopamine, Tyramine, or DA-quinone treatment induce mitochondrial fragmentation, decrease p-Ser637 DRP1 and increase DRP1 mitochondrial localization. A SH-SY5Y cells are treated with DA (200 µM), Tyramine (1 mM) or DAQ (200 µM co-incubation with Tyrosinase) for 16 h and mitochondria is visualized by immunostaining for ATP5a. Scale bar 10 µm. B Mitochondrial morphology is analyzed after the treatment as mentioned above, and mean proportion of filamentous, rod shaped or punctate mitochondria is represented in the graphs. At least 30 cells are analyzed from 3 independent experiments for each group. C SH-SY5Y cells are treated as mentioned in A and total cell lysate is subjected to SDS PAGE followed by immunoblotting for the mentioned proteins. Immunoblots are representative of at least 3 different experiments. D Mean normalized band intensities of the mentioned proteins in C are represented as bar graphs. E SH-SY5Y cells are treated as mentioned in A and protein lysate from mitochondria is subjected to SDS PAGE followed by immunoblotting for the mentioned proteins. Immunoblots are representative of at least 3 different experiments. F Mean normalized band intensities of the mentioned proteins in E are represented as bar graphs. Bar graphs represent mean ± SEM. N = at least 3 for each group. P values are calculated by Student’s t test. *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001 compared to control group