Fig. 4

TNFα treatment rescues impaired osteoclastogenesis induced by Fanca KO. A Number of OCs for field (left), number of nuclei for OC (middle) and surface of the OCs (right) from the WT and Fanca^−/− mice at day 4 of differentiation after TNFα treatment (10 ng/ml). B Relative Nfatc1, Dc-Stamp and Cathepsin K expression evaluated by qRT-PCR in progenitors, OCs and OCs treated with TNFα at day 4 of differentiation from the WT and Fanca^−/− mice. C Left: Western blot showing Nfatc1 and Cathepsin K in OCs from WT and Fanca^−/− mice at day 4 of differentiation that were left untreated or treated with TNFα. β-Actin was used as a loading control. Right: Relative protein expression of Nfatc1 and Cathepsin K in OCs from WT and Fanca^−/− mice at day 4 of differentiation that were left untreated or treated with TNFα. D Relative Cdkn1a expression evaluated by qRT-PCR in progenitors, OCs and OCs treated with TNFα at day 4 of differentiation from WT and Fanca^−/− mice. Data are shown as the mean ± SEM. Each small circle represents an individual mouse. Statistical analysis was performed by t tests: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001