Fig. 3
From: Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα
p53–p21 participates in impaired osteoclastogenesis induced by Fanca KO. A Relative Cdkn1a expression evaluated by qRT-PCR in progenitors, OCs and OCs from WT and Fanca−/− mice treated with Nutlin-3 or pifithrin-α (Nut and Pif, 1 μM each) at days 4 and 5 of differentiation. B Number of OCs for field (left), number of nuclei for OC (middle) and surface of the OCs (right) from the WT and Fanca−/− mice at day 5 of differentiation after Nutlin-3 (Nut) or Pifithrin-α (Pif) treatment (1 μM each). C–E Relative Nfatc1, DC-Stamp and cathepsin K expression evaluated by qRT-PCR in progenitors, OCs and OCs from Fanca−/− mice treated with Nutlin-3 or pifithrin-α (Nut and Pif, 1 μM each) at days 4 and 5 of differentiation. Data are shown as the mean ± SEM. Each small circle represents an individual mouse. Statistical analysis was performed by t tests: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001