Fig. 2

Fanca deficiency leads to impaired osteoclastogenesis at the molecular level. A Relative Rank and M-Csfr expression evaluated by qRT-PCR in progenitors and OCs from WT and Fanca^−/− mice at day 4 of differentiation. B Relative Nfatc1 expression evaluated by qRT-PCR in progenitors and OCs from WT and Fanca^−/− mice at day 4 of differentiation. C Relative Oscar, Dc-Stamp, Atp6v0d2, cathepsin K and TRAP expression evaluated by qRT-PCR in progenitors and OCs from WT and Fanca^−/− mice at day 4 of differentiation. D Representative Western blot showing Nfatc1 and Cathepsin K in OCs from WT and Fanca^−/− mice at days 4 and 5 of differentiation. Relative protein expression of Nfatc1 and Cathepsin K in WT and Fanca^−/− mice at days 4 and 5 of differentiation. β-Actin was used as a loading control. Data are shown as the mean ± SEM. Each small circle represents an individual mouse. Statistical analysis was performed by t tests: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001