Fig. 1

Fanca deficiency is associated with altered osteoclastogenesis. A Representation of the strategy used to differentiate OCs from isolated BM cell populations. B TRAP staining of OCs from WT and Fanca^−/− mice at day 5 of differentiation. C Representative images of F-actin IF in OCs from WT and Fanca^−/− mice at day 5 of differentiation. D Number of OCs per field (left), number of nuclei for OC (middle) and surface of the OCs (right) at days 3, 4 and 5 of differentiation. E Representative images after TRAP staining of OCs from WT and Fanca^−/− mice at day 4 of differentiation after treatment with the indicated doses of RANKL. F Number of OCs per field (left), number of nuclei for OC (middle) and surface of the OCs (right) at day 4 of differentiation after treatment with the indicated doses of RANKL. Data are shown as the mean ± SEM. Each small circle represents an individual mouse. Statistical analysis was performed by t tests: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001