Fig. 6

TMCO1 knockdown sensitizes IRE1α-XBP1 axis to promote cell survival. a Representative annexin V-FITC and PI staining in WT or TMCO1 KD cells treated with/without 20 μM KIRA6 for 24 h followed by flow cytometry. AV, annexin V. b The proportion of dead cells was counted in A. Bar graphs represent the mean ± SEM from three independent assays. **P < 0.01, ***P < 0.001. c Western blotting analysis of proteins in WT and TMCO1 KD cells treated with/without 20 μM KIRA6 for 24 h. GAPDH was used as a loading control. d qRT-PCR analysis of Xbp1s in the liver from Tmco1^+/+ and Tmco1^−/−mice at 2 months of age injected intraperitoneally with TM (2 μg/g) for 8 h. Each point represents independent animal. Untreated mice, NT: n = 8; TM treated mice: n = 8. *P < 0.05, n.s., no significance. e The levels of XBP1s in the livers from Tmco1^+/+ and Tmco1^−/− mice injected intraperitoneally with TM (2 μg/g) for 8 h were measured through immunoblotting. GAPDH was used as a loading control. Quantification of the relative expression of XBP1s was shown in the right panel. Bar graphs represent the mean ± SEM from three independent experiments. Each point represents independent animal. *P < 0.05. f qRT-PCR analysis of Bloc1s1, Bip, Chop in the liver from Tmco1^+/+ and Tmco1^−/−mice injected intraperitoneally with TM (2 μg/g) for 8 h. Each point represents independent animal. Untreated mice, NT: n = 8; TM treated mice: n = 8. n.s., no significance. g Representative images of H&E staining of liver tissues from Tmco1^+/+ and Tmco1^−/− mice injected with Tm for 8 h (magnification × 200). Scale bar, 100 μm. Three animals per group were analyzed