Fig. 3

Serine protease PRSS56 is a novel CT antigen that reactivated by DNA methyltransferase inhibitor. (A and B) The RNA sequencing studies (GSE215214) were conducted in AGS and HCT116 cell lines exposure to the DNA methyltransferase inhibitor 5-Aza-CdR (5 µM). The Gene expression fold changes (log2FC value) were shown in the volcano plots. (C) The genes with the most significant expression fold changes (log2FC > 1) after 5-Aza-CdR treatment were displayed in the heat map. (D) There were nine genes with the most significant upregulation (log2FC > 2) after 5-Aza-CdR treatment were shown in the plot. (E) PRSS56 was a novel CT antigen that activated by 5-Aza-CdR. (F, G) RNA-seq analysis and qRT-PCR assay together confirmed that PRSS56 was reactivated by 5-Aza-CdR in both AGS and HCT116 cell lines