Fig. 6

TRIM67 suppressed the activation of the NF-κB signaling pathway induced by cerebral ischemia‒reperfusion injury. A In vivo study of essential kinases in the NF-κB pathway using western blotting. B–G Quantitative analysis of p-IKKα/β, total-IKKα/β, p-IκBα, total-IκBα, p-p65 and total-p65 are shown in A (n = 5). H In vivo western blotting investigation of NF-κB p65 levels in the cytoplasm and nucleus. I and J NF-κB p65 quantitative analysis is displayed in (H) (n = 3). K NF-κB p65 expression in mice exposed to MCAO/R as seen in immunofluorescence images, DAPI (blue)/NF-κB p65 (green). Scale bar: 40 μm. L The levels of NF-κB p65 in the cytoplasm and nucleus were quantified. The mean ± SD are displayed for all data. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001